I have an assay questions 1. Compare between monoclonal and polyclonal antibodi

I have an assay questions
1. Compare between monoclonal and polyclonal antibodies.
2. Compare between nephlometry and florometry
3. Enumerate the different methods of labelling in immunoassay and its
specificity.
4. Compare between direct ELISA and indirect ELISA
5. What are the factors affecting sandwich ELISA?
you have to explain and include references